CRISPR Rat Deletion INS-1 832/13

Get tips on using β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer to perform Reporter gene assay β-galactosidase substrates - SK-Hep-1

Get tips on using Purified Mouse Anti-p62 Ick ligand Clone 3/P62 LCK LIGAND (RUO) to perform Autophagy assay cell type - THP 1

Get tips on using Lipofectamine® 2000 Transfection Reagent to perform siRNA / miRNA gene silencing Human - Primary Endometrial Stromal Cells IGFBP1 (Insuline-like growth factor binding protein-1) Lipid

Get tips on using Anti-CD133 Antibody, clone 13A4 to perform Immunohistochemistry Mouse - CD133

Get tips on using Anti-Glucagon Antibody, clone 13D11.33 to perform Immunohistochemistry Mouse - Glucagon

Get tips on using ATP5A Antibody (51): sc-136178 to perform Western blotting ATP5A

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using p-NOS3 Antibody (pT495.33): sc-136519 to perform Western blotting eNOS

Get tips on using Progerin Antibody (13A4D4): sc-81611 to perform Western blotting Lamin A/C

Get tips on using MMP-2 Antibody (2C1): sc-13594 to perform Western blotting MMP-2