Get tips on using DNeasy PowerBiofilm Kit (50) to perform DNA isolation / purification Biofilm samples
Get tips on using DNeasy Blood & Tissue Kit to perform DNA isolation / purification Tissue - adipose
Get tips on using DNeasy Blood & Tissue Kit to perform DNA isolation / purification Tissue - placenta
Get tips on using DNeasy Blood & Tissue Kit to perform DNA isolation / purification Tissue - eye
Get tips on using DNeasy Blood & Tissue Kit to perform DNA isolation / purification Tissue - lung
Get tips on using DNeasy Blood & Tissue Kit to perform DNA isolation / purification Tissue - brain
Get tips on using DNeasy PowerLyzer PowerSoil Kit (100) to perform DNA isolation / purification Insects
Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.
Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.
Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.
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