Protein quantification Mammalian cells

Get tips on using DMEM/F-12 to perform Mammalian cell culture media U251

Get tips on using RPMI-1640 Medium to perform Mammalian cell culture media U937

Get tips on using DULBECCO MEM MEDIUM to perform Mammalian cell culture media U87

Get tips on using DULBECCO MEM MEDIUM to perform Mammalian cell culture media T98G

Get tips on using DMEM/F-12 to perform Mammalian cell culture media T47D

Get tips on using RPMI-1640 Medium to perform Mammalian cell culture media PNT1A

Get tips on using DMEM/F-12 to perform Mammalian cell culture media A2780

Get tips on using CD CHO Medium to perform Mammalian cell culture media CHO

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.