Protein Expression Eukaryotic cells S. cerevisiae

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Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,

DNA DNA isolation / purification Cells Immortalized cell lines HEK 293T

Cell culture media 3D Cell Culture Media PLC/PRF/5 cells-spheres

DNA DNA isolation / purification Cells Immortalized cell lines Loucy

DNA DNA isolation / purification Cells Immortalized cell lines MKN45

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

Cellular assays ROS assay cell type human umbelical vein endothelial cells (HUVEC)

Cell culture media 3D Cell Culture Media BT-549 cells-Mammospheres

Get tips on using GeneChip™ Hybridization, Wash, and Stain Kit to perform Microarray Gene expression arrays - Rat mesothelium Satin cocktail

Products Thermo Fisher Scientific GeneChip™ Hybridization, Wash, and Stain Kit
Best Antibody Discussion

which is a good monoclonal anibody for this protein?

Discussions Best Antibody

Get tips on using GeneChip™ Rat Genome 230 2.0 Array to perform Microarray Gene expression arrays - Rat mesothelium Satin cocktail

Products Thermo Fisher Scientific GeneChip™ Rat Genome 230 2.0 Array

Get tips on using Streptavidin-R-PE to perform Protein tag Detection of biotinylated proteins

Products Qiagen Streptavidin-R-PE

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