rna-isolation-purification-tissue-rat-bronchi

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized A431

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized A2780ADR

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized A2780

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized A253

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized CHO

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized MDCK

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized CMT12

Get tips on using RNeasy PowerPlant Kit (50) to perform RNA isolation / purification Plants - Seeds

Get tips on using RNeasy PowerWater Kit (50) to perform RNA isolation / purification Water samples

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.