siRNA / RNAi /miRNA transfection Human Primary splenocytes

Get tips on using RosetteSep™ Human T Cell Enrichment Cocktail to perform Cell Isolation Human T cells

Get tips on using MagniSort™ Human T cell Enrichment Kit to perform Cell Isolation Human T cells

Get tips on using EasySep™ Human T Cell Enrichment Kit to perform Cell Isolation Human T cells

Get tips on using EasySep™ Human T Cell Isolation Kit to perform Cell Isolation Human T cells

Get tips on using Human TGF beta 1 ELISA Kit (ab100647) to perform ELISA Human - TGF-beta 1

Get tips on using Human TGF-beta 1 Quantikine ELISA Kit to perform ELISA Human - TGF-beta 1

Get tips on using Human IL-1 beta ELISA Kit (ab100562) to perform ELISA Human - IL-1 beta

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using PE anti-human CD114 (G-CSFR) Antibody to perform Flow cytometry Anti-bodies Human - CD114