Get tips on using DMEM:F12 with HEPES, Glucose and L-Glutamine to perform 3D Cell Culture Media Human lung cancer organoids
Get tips on using GenElute™ Mammalian Total RNA Miniprep Kit to perform RNA isolation / purification Cells - immortalized human pancreatic cancer
Get tips on using Phusion Site-Directed Mutagenesis Kit to perform Site Directed Mutagenesis (SDM) Human - Deletion MCF-7 sodium channel β1 subunit
Get tips on using Global DNA Methylation ELISA to perform DNA methylation profiling Whole genome profiling - HCT116, HTC15 human colon cancer cells
Get tips on using CpGenome Universal DNA Modification Kit to perform DNA methylation profiling Whole genome profiling - OVCAR-3 human ovarian cancer
DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.
DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.
Get tips on using In Situ Cell Death Detection Kit, Fluorescein to perform TUNEL assay cell type - SKOV3, Caov3 human ovarian cancer
Get tips on using in situ Cell Death Detection Kit, POD to perform TUNEL assay cell type - PANC-1 human pancriatic cancer
Get tips on using In Situ Cell Death Detection Kit, Fluorescein to perform TUNEL assay cell type - PC-3 human prostate cancer
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