DNA methylation profiling Gene specific profiling HEK 293T

Get tips on using ON-TARGETplus Human CDK2 (1017) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - HEK293 CDK2

Get tips on using ON-TARGETplus Human SLC7A2 (6542) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - HEK293 SLC7A2

Get tips on using ON-TARGETplus Human SLC4A2 (6522) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - HEK293 SLC4A2

Get tips on using ON-TARGETplus Human BEST1 (7439) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - HEK293 BEST1

Get tips on using ON-TARGETplus Human CLCN3 (1182) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - HEK293 CLCN3

Get tips on using ON-TARGETplus Human ANO1 (55107) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - HEK293 ANO1

Get tips on using Gentra Puregene Cell Kit Plus (6.7 x 109) to perform DNA isolation / purification Cells - Immortalized cell lines H1 hESc

Plasmid isolation is an important technique in molecular biology or any kind of genetic editing. It involves amplifying plasmids overnight by transforming them into competent bacterial cells. The desired colonies of these bacteria can then be grown in shaker cultures, at appropriate shaking speed, oxygen availability and temperature. These liquid cultures can then be ultracentrifuged to pellet the bacteria, which are then used for plasmid isolation. The bacteria are first resuspended in a buffer, then lysed, neutralized, purified in a column, eluted, precipitated with ethanol and then resuspended. During plasmid isolation, it is important to lyse cells quickly because lysing bacteria for too long may lead to irreversible denaturing of the plasmid. Usually, alkaline lysis is used for isolation because it is a mild treatment. It isolates plasmid DNA and other cell components such as proteins by breaking cells apart with an alkaline solution. Precipitation removes the proteins, and the plasmid DNA recovers with alcohol precipitation. Resuspension and lysis buffers should be mixed thoroughly in order to prevent the DNA from breaking into smaller fragments. This is because broken gDNA can reanneal and remain in the solution, without binding to the column.

Get tips on using Accell Human VDAC1 (7416) siRNA - Set of 4 to perform siRNA / miRNA gene silencing Human - HEK293 VDAC1

Get tips on using GeneBLAzer In Vivo Detection Kit to perform Reporter gene assay β-lactamase substrates - HeLa