DNA methylation profiling Gene specific profiling Rat whole pituitary glands

Get tips on using MMP-3 (D7F5B) Rabbit mAb #14351 to perform Immunohistochemistry Rat - MMP3

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using lentiCRISPR v2 to perform CRISPR Rat - Deletion PC12 MMP9

Get tips on using EZ-ChIP™ to perform ChIP Rat - Mesenteric arteries

Get tips on using Anti-SOX9 Antibody AB5535 to perform Immunohistochemistry Rat - Sox9

Get tips on using Anti-GFAP antibody (ab7260) to perform Immunohistochemistry Rat - GFAP

Get tips on using Anti-MMP3 antibody (ab53015) to perform Immunohistochemistry Rat - MMP3

Get tips on using ChromaFlash Chromatin Extraction Kit to perform ChIP Rat - Heart

Get tips on using EpiTect ChIP qPCR Assays to perform ChIP Rat - Brain

Get tips on using HSP70 ELISA Kit (ab133060) to perform ELISA Rat - HSP70