siRNA / miRNA gene silencing Rat RGC-5

Get tips on using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005 to perform ChIP Rat - Pancreas

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Get tips on using FuGENE® 6 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Rat aortic smooth muscle cells (rASMC)

Get tips on using Ambion™ RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE to perform RNA isolation / purification Tissue - rat kidney tissue

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary rat pulmonary artery smooth muscle cells

Get tips on using Absolutely RNA Nanoprep Kit to perform RNA isolation / purification Cells - primary rat dorsal root ganglion neurons

Get tips on using Cell Death Detection ELISA to perform TUNEL assay cell type - Rat pulmonary arterial smooth muscle cells

Get tips on using Lipofectamine® 2000 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Rat mesangial cells

Get tips on using FuGENE® HD Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Rat mesangial cells

Get tips on using FuGENE® 6 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Rat articular chondrocytes