siRNA / miRNA gene silencing Rat NRK

Get tips on using EZViable™ Calcein AM Cell Viability Assay Kit (Fluorometric) to perform Live / Dead assay mammalian cells - rat brain microvascular endothelial cells

Get tips on using LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells to perform Live / Dead assay mammalian cells - rat tendon-derived stem cells

Get tips on using Image-IT™ LIVE Green Reactive Oxygen Species Detection Kit, for microscopy to perform ROS assay cell type - H9c2 rat cardiomyocytes

Get tips on using Live or Dead™ Cell Viability Assay Kit *Green/Red Dual Fluorescence to perform Live / Dead assay mammalian cells - rat endothelial progenitor cells

Get tips on using GeneChip Rhesus Macaque Genome Array to perform Microarray Gene expression arrays - Rhesus monkey brain tissue Biotin

Get tips on using LIVE/DEAD™ Fixable Near-IR Dead Cell Stain Kit, for 633 or 635 nm excitation to perform Live / Dead assay mammalian cells - rat testicular tissue

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using pX330-U6-Chimeric_BB-CBh-hSpCas9 to perform CRISPR Mouse - Deletion RAW 264.7 Nrp2

Get tips on using EZ DNA Methylation-Direct Kit to perform DNA methylation profiling Gene specific profiling - A54 RASSF1A

Get tips on using Quick Amp Labeling Kit-one color to perform RNA amplification & labeling Mammalian - miRNA, Human Endometrial Stromal cells Cyanine 3-pCp