CRISPR Mouse Activation 3T3-L1

Get tips on using Notch 1 Antibody (E-4): sc-373944 to perform Western blotting Notch1

Get tips on using ON-TARGETplus Human IFIT1 (3434) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - HFF1 IFIT1

Get tips on using ON-TARGETplus Human IFIT2 (3433) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - HFF1 IFIT2

Get tips on using ON-TARGETplus Rat Arhgef6 (363509) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - MTLn3 α-Pix/Arhgef6

Get tips on using ON-TARGETplus Rat Rac1 (363875) siRNA - Set of 4 to perform siRNA / miRNA gene silencing Rat - MTLn3 Rac1

Get tips on using Hoechst 33258 to perform DNA quantification Human - MDA-MB-231

Get tips on using beta Tubulin Monoclonal Antibody (2 28 33) to perform Western blotting Tubulin beta

Get tips on using Phospho-eIF2α (Ser51) (D9G8) XP® Rabbit mAb #3398 to perform Autophagy assay cell type - HEK 293

Get tips on using D,L-Sulforaphane N-Acetyl-L-cysteine (SFN-NAC) (CAS 334829-66-2) to perform Autophagy assay cell type - U373MG

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.