RNA isolation / purification Cells Cancer cell lines Leukemia cancer cell lines

Get tips on using RNAzol® RT to perform RNA isolation / purification Cells - immortalized DU145

Get tips on using Dynabeads™ Untouched™ Human B Cells Kit to perform Cell Isolation B cell

Get tips on using NucleoSpin® miRNA to perform RNA isolation / purification Cells - primary canine aortic endothelial cells

Get tips on using NucleoSpin® miRNA to perform RNA isolation / purification Cells - primary human mesenchymal stem cells

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - primary human endometrial stromal cells

Get tips on using mirVana® miRNA mimic to perform RNA isolation / purification Cells - primary human endothelial cells

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using Wizard® Genomic DNA Purification Kit to perform DNA isolation / purification Cells - Primary cells HUVEC

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human pulmonary arterial smooth muscle cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary mouse pulmonary artery smooth muscle cells