Get tips on using pX330-U6-Chimeric_BB-CBh-hSpCas9 to perform CRISPR Mouse - Deletion 3T3-L1 MmP13
Get tips on using pX330-U6-Chimeric_BB-CBh-hSpCas9 to perform CRISPR Mouse - Deletion ES (embryonic stem) cells Slx2
The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.
Get tips on using hCas9 to perform CRISPR Human - Deletion Hsp90α
Get tips on using Edit-R CRISPR-Cas9 Nuclease Expression Plasmid to perform CRISPR Mouse - Repression XylT2
Get tips on using lentiCRISPR v2 to perform CRISPR Human - Deletion CD38
Get tips on using lentiGuide-Puro to perform CRISPR Human - Deletion CLK1
Get tips on using pLX-sgRNA to perform CRISPR Human - Deletion CIITA
Get tips on using lentiCRISPR v2 to perform CRISPR Human - Deletion AXL
Get tips on using lentiCRISPR v2 to perform CRISPR Human - Deletion NGRN
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