Site Directed Mutagenesis (SDM) Human Point mutation U2OS

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Get tips on using ON-TARGETplus SMARTpool - Human to perform siRNA / miRNA gene silencing Human - U2OS DKC1

Get tips on using ON-TARGETplus Human BLM (641) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - U2OS BLM

Get tips on using ON-TARGETplus Human MUS81 (80198) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - U2OS MUS81

Get tips on using ON-TARGETplus Human SLX1A (548593) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - U2OS SLX1A

Get tips on using ON-TARGETplus Human SLX4 (84464) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - U2OS SLX4

Get tips on using ON-TARGETplus Human ERCC4 (2072) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - U2OS XPF/ERCC4

Get tips on using NM_001350 to perform siRNA / miRNA gene silencing Human - U2OS DAXX

Get tips on using AllStars Hs Cell Death siRNA to perform siRNA / miRNA gene silencing Human - U2OS KRAS

Get tips on using Atg5 (D5F5U) Rabbit mAb to perform Autophagy assay cell type - U2OS (human bone osteosarcoma epithelial cells)