Protein Expression Prokaryotic cells C. glutamicum

Microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Get tips on using TAGZyme Kit to perform Protein tag His-tag removal

Get tips on using Trichloroacetic acid to perform Protein isolation Bacteria - Bacillus subtilis

Get tips on using Trichloroacetic acid to perform Protein isolation Bacteria - Bacillus anthracis

Get tips on using Qproteome Mitochondria Isolation Kit to perform Protein enrichment Mitochondria

Get tips on using Ni-NTA HisSorb Strips (24) to perform Protein tag Detection of His-tagged proteins