Site Directed Mutagenesis (SDM) Mouse Point mutation C2C12

Get tips on using Estrogen Receptor alpha Antibody (F-10): sc-8002 to perform Immunohistochemistry Mouse - ERα

Get tips on using Recombinant Anti-VEGFA antibody [EP1176Y] - C-terminal (ab52917) to perform Immunohistochemistry Mouse - VEGFA

Get tips on using Anti-phospho-Histone H3 (Ser10) Antibody, Mitosis Marker to perform Immunohistochemistry Mouse - PHH3

Get tips on using truChIP Ultra-Low Chromatin Shearing Kit with Formaldehyde to perform ChIP Mouse - Osteoblasts

Get tips on using EZ-Magna ChIP™ G - Chromatin Immunoprecipitation Kit to perform ChIP Mouse - Ovary

Get tips on using EZ-Magna ChIP™ A - Chromatin Immunoprecipitation Kit to perform ChIP Mouse - Podocyte

Get tips on using EZ-Magna ChIP™ HiSens Chromatin Immunoprecipitation Kit to perform ChIP Mouse - HSC

Get tips on using Gibco™Advanced DMEM/F-12 to perform 3D Cell Culture Media Mouse small intestinal organoids

Get tips on using HTRA2 MISSION shRNA Lentiviral Transduction Particles HtrA serine peptidase 2 to perform shRNA gene silencing Mouse - FL83B HtrA2

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. However, non-specific product amplification and primer-dimer formation during set-up are major causes of PCR failure. Nevertheless, high-quality hot-start DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction