Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary rabbit skeletal muscle cells
Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary rat marrow stromal cells
As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.
Get tips on using illustra RNAspin Mini Kit to perform RNA isolation / purification Cells - immortalized BRL 3A
Get tips on using RNAprep pure Kit (For Plant) to perform RNA isolation / purification Yeast - Neurospora crassa
Though DNA quantification is but one small step in the multifaceted DNA sample preparation workflow, it can have large implications on the performance and validity of conclusions drawn from downstream assays. Major challenges include accuracy, precision, reproducibility, and detection of present contamination. Among UV spectrophotometry, fluorescence and real-time PCR based methods, the quantification method should be chosen based on the requirement of the downstream assay.
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human airway epithelial cells
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human peripheral blood cells
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human mammary mesenchymal cells
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human mammary epithelial cells
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