Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase
Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase
Get tips on using DMEM HIGH GLUCOSE to perform Mammalian cell culture media HFLS-OA
Get tips on using MCDB 131 Medium to perform Mammalian cell culture media CADMEC/HMVEC
Get tips on using RPMI-1640 Medium to perform Mammalian cell culture media HL-60
Get tips on using DMEM/F-12 to perform Mammalian cell culture media MCF-10A
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