Site Directed Mutagenesis (SDM) Human Point mutation BxPC-3

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RNA siRNA / miRNA gene silencing Human U937 MK2 (MAPK Kinase 2) Viral vectors

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

RNA RNA isolation / purification Cells primary human hair follicle dermal papilla cells

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel

RNA RNA isolation / purification Cells primary human renal proximal tubular epithelial cells

Cellular assays Cell line authentication Human prostatic cancer cell line PC3

Cellular assays Cell line authentication Human prostatic cancer cell line DU145

Get tips on using EasySep™ Human B Cell Enrichment Kit II Without CD43 Depletion to perform Cell Isolation B cell

Products STEMCELL technologies EasySep™ Human B Cell Enrichment Kit II Without CD43 Depletion

Get tips on using Purified Mouse Anti-Human ZO-1 Clone 1/ZO-1 (RUO) to perform Western blotting ZO-1

Products BD Biosciences Purified Mouse Anti-Human ZO-1 Clone 1/ZO-1 (RUO)

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

Cellular assays ROS assay cell type BEAS-2B human bronchial epithelial cell line

Get tips on using Direct-zol RNA Kits to perform RNA isolation / purification Cells - immortalized PC-3

Products Zymo Research Direct-zol RNA Kits

DNA ladder is typically used as a reference to estimate the size of unknown DNA samples that are separated based on their mobility in an electrical field. The critical points for running a DNA ladder are compatibility with running buffer, agarose gel percentage, and choosing the correct range of DNA ladder for sizing DNA molecules.

DNA DNA Ladder 1 kb

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