Protein quantification Mammalian cells

Get tips on using CnAOEC Growth Medium Kit to perform Mammalian cell culture media CnAOEC

Get tips on using Minimum Essential Medium Eagle to perform Mammalian cell culture media BAOSMC

Get tips on using MEM (Eagle) Liquid Medium to perform Mammalian cell culture media MDCK

Get tips on using Minimum Essential Media (MEM) to perform Mammalian cell culture media MDCK

Get tips on using Minimum Essential Media (MEM) to perform Mammalian cell culture media Vero

Get tips on using Minimum Essential Medium Eagle to perform Mammalian cell culture media Vero

Get tips on using Complete Kit for Human Whole Blood CD34+ Cells to perform Cell Isolation CD34+ cells

Get tips on using Dynabeads™ Untouched™ Human T Cells Kit to perform Cell Isolation Human T cells

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.