siRNA / miRNA gene silencing Human ES2(ovarian cancer cell line)

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

Get tips on using CytoSelect™ 24-Well Cell Migration Assay, 3 µm to perform Cell migration / Invasion cell type - isolated human neutrophils

Get tips on using GeneChip® Human Genome U133 Plus 2.0 Array to perform Microarray Human - Endometrial stromal cells Expression array

Get tips on using Human Synoviocyte Growth Medium to perform Mammalian cell culture media HFLS-OA

Get tips on using Human Apoptosis Array G1 to perform Apoptosis assay cell type - PC-3

Get tips on using Guava Cell Cycle Reagent for Flow Cytometry to perform Cell cycle assay human - OVCAR-5

Get tips on using Guava Cell Cycle Reagent for Flow Cytometry to perform Cell cycle assay human - THP-1

Get tips on using Guava Cell Cycle Reagent for Flow Cytometry to perform Cell cycle assay human - MCF-7

Get tips on using Guava Cell Cycle Reagent for Flow Cytometry to perform Cell cycle assay human - SH-SY5Y

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.