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Cell Culture Contamination Detection Kit Yeast Experiment

Contamination can affect cell characteristics, i.e., growth, metabolism, and morphology leading to unreliable and erroneous experimental data. Depending on the source of contaminants, one can detect contamination by using a light microscope, gram stain, isothermal amplification, or PCR. Bacteria and fungi can usually be identified by optical microscopy. Mycoplasma in cell cultures cannot be detected visually. Hence, these microbes can go unnoticed for long periods and are determined using dedicated assays. Early and rapid identification of contaminants is vital to detect, handle and prevent contamination for good cell-culture practices. However, detection and identification can be challenging and tricky based on usual visual identifications. Hence it is essential to use a standard contamination detection kit to detect and maintain best practices.

Cellular assays Cell Culture Contamination Detection Kit Yeast

RNA isolation / purification Cells - primary human blood endothelial cell Experiment

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

RNA RNA isolation / purification Cells primary human blood endothelial cell

Senescence Cells Histochemical Staining Kit Product

Get tips on using Senescence Cells Histochemical Staining Kit to perform Cell cycle assay mouse - L929

Products Sigma-Aldrich Senescence Cells Histochemical Staining Kit

PI/RNase Staining Buffer Product

Get tips on using PI/RNase Staining Buffer to perform Cell cycle assay mouse - C2C12