RNA isolation / purification

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Purelink PCR Purification Kit Product

Get tips on using Purelink PCR Purification Kit to perform DNA gel extraction / PCR product purification Product size < 15Kb

Products Thermo Fisher Scientific Purelink PCR Purification Kit

QIAquick PCR Purification Kit Product

Get tips on using QIAquick PCR Purification Kit to perform DNA gel extraction / PCR product purification Product size < 15Kb

Products Qiagen QIAquick PCR Purification Kit

Cell Isolation PBMC Isolation Experiment

Cellular assays Cell Isolation PBMC Isolation

PureLink™ HiPure Plasmid Filter Purification Kits - for Midi and Maxi preparation of Plasmid DNA Product

Get tips on using PureLink™ HiPure Plasmid Filter Purification Kits - for Midi and Maxi preparation of Plasmid DNA to perform Plasmid Isolation Citrobacter koseri

Products Thermo Fisher Scientific PureLink™ HiPure Plasmid Filter Purification Kits - for Midi and Maxi preparation of Plasmid DNA

Plasmid Isolation Enterobacteriaceae Experiment

Plasmid isolation is an important technique in molecular biology or any kind of genetic editing. It involves amplifying plasmids overnight by transforming them into competent bacterial cells. The desired colonies of these bacteria can then be grown in shaker cultures, at appropriate shaking speed, oxygen availability and temperature. These liquid cultures can then be ultracentrifuged to pellet the bacteria, which are then used for plasmid isolation. The bacteria are first resuspended in a buffer, then lysed, neutralized, purified in a column, eluted, precipitated with ethanol and then resuspended. During plasmid isolation, it is important to lyse cells quickly because lysing bacteria for too long may lead to irreversible denaturing of the plasmid. Usually, alkaline lysis is used for isolation because it is a mild treatment. It isolates plasmid DNA and other cell components such as proteins by breaking cells apart with an alkaline solution. Precipitation removes the proteins, and the plasmid DNA recovers with alcohol precipitation. Resuspension and lysis buffers should be mixed thoroughly in order to prevent the DNA from breaking into smaller fragments. This is because broken gDNA can reanneal and remain in the solution, without binding to the column.

DNA Plasmid Isolation Enterobacteriaceae

High Pure PCR Product Purification Kit Product

Get tips on using High Pure PCR Product Purification Kit to perform DNA gel extraction / PCR product purification Product size > 15Kb

Products Roche Lifesciences High Pure PCR Product Purification Kit

Agencourt AMPure XP - PCR Purification Product

Get tips on using Agencourt AMPure XP - PCR Purification to perform

Products Beckman Coulter Agencourt AMPure XP - PCR Purification

RNeasy 96 Kit (12) Product

Get tips on using RNeasy 96 Kit (12) to perform mRNA / Ribonucleoprotein isolation / purification Ribonucleoprotein

Products Qiagen RNeasy 96 Kit (12)

Cell Isolation Double-negative T Cell Isolation Experiment

Cellular assays Cell Isolation Double-negative T Cell Isolation

AllPrep DNA/RNA/Protein Mini Kit Product

Get tips on using AllPrep DNA/RNA/Protein Mini Kit to perform Protein isolation Tissue - Human placental tissue

Products Qiagen AllPrep DNA/RNA/Protein Mini Kit

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