dna-methylation-profiling-gene-specific-profiling-siha-hpv-16

- Found 4875 results

RNA isolation / purification Cells - primary human pancreatic stellate cells Experiment

The process of RNA extraction from bacteria, in general, involves an RNA-protective, effective lysis of bacterial cell wall (which may pose difficulties). EDTA promotes loss of outer membrane to provide lysozyme with access to peptidoglycan. Another common method for cell wall lysis is mechanical disruption using a homogenizer (applied for gram-positive bacteria and some strains of gram-negative bacteria). Following lysis, it is necessary to disrupt protein-nucleic acid interactions, which can be achieved by adding sodium dodecyl sulfate (SDS). Next step involves using phenol-chloroform-isoamyl alcohol extraction, where RNA can be obtained from the bottom organic phase, the top phase consists of DNA and the interphase contains proteins. Isoamyl alcohol is an inert and optional addition to this mixture and is added as an anti-foaming reagent to reduce the interphase. Following RNA extraction, the samples should be checked for its quality by gel electrophoresis (23S and 16S rRNAs and 5s rRNA and tRNA bands) or UV spectrophotometric or fluorescence methods.

RNA RNA isolation / purification Cells primary human pancreatic stellate cells

DNeasy PowerWater Kit (100) Product

Get tips on using DNeasy PowerWater Kit (100) to perform DNA isolation / purification Water samples

Products Qiagen DNeasy PowerWater Kit (100)

DNeasy PowerBiofilm Kit (50) Product

Get tips on using DNeasy PowerBiofilm Kit (50) to perform DNA isolation / purification Biofilm samples

Products Qiagen DNeasy PowerBiofilm Kit (50)

DNeasy Blood & Tissue Kit Product

Get tips on using DNeasy Blood & Tissue Kit to perform DNA isolation / purification Tissue - adipose