DNA methylation profiling Gene specific profiling SKOV3

Get tips on using Gentra Puregene Blood Kit to perform DNA isolation / purification Tissue - blood / plasma

Get tips on using QIAGEN Genomic-tip 20/G to perform DNA isolation / purification Plants - Leaves

Get tips on using siRNA MXD3 to perform siRNA / miRNA gene silencing Human - DAOY MXD3

Get tips on using siRNA FOXS1 to perform siRNA / miRNA gene silencing Human - DAOY FOXS1

Get tips on using DNeasy UltraClean Microbial Kit to perform DNA isolation / purification Bacteria - Gram positive Pseudomonas

Get tips on using DNeasy Blood & Tissue Kit to perform DNA isolation / purification Cells - Primary cells HUVEC

Get tips on using DNeasy Blood & Tissue Kit to perform DNA isolation / purification Bacteria - Gram negative Legionella

Get tips on using DNeasy Plant Pro Kit (250) to perform DNA isolation / purification Plants - Mycelial mats

Get tips on using DNeasy PowerClean Pro Cleanup Kit (50) to perform DNA isolation / purification Water samples

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.