siRNA / miRNA gene silencing Human ES2(ovarian cancer cell line)

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

Get tips on using RosetteSep™ Human Monocyte Enrichment Cocktail to perform Cell Isolation Monocyte

Get tips on using EasySep™ Human Monocyte Enrichment Kit to perform Cell Isolation Monocyte

Get tips on using EasySep™ Human Monocyte Isolation Kit to perform Cell Isolation Monocyte

Get tips on using GeneChip® Human Genome U133 Plus 2.0 Array to perform RNA amplification & labeling Mammalian - RNA amplification and Labeling Human Endometrial Stromal cells Biotin

Get tips on using GeneChip® Human Genome U133 Plus 2.0 Array to perform RNA amplification & labeling Mammalian - RNA, rhesus monkey brain tissue Human endothelail stromal cells

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using GeneChip™ Human Genome U133A 2.0 Array to perform Microarray Comperative genomic hybridization - Human Tumor

Get tips on using MammoCult™ Human Medium Kit to perform Stem cell culture media hMammospheres

Get tips on using Anti-p62 (SQSTM1) (Human) pAb to perform Autophagy assay cell type - A549