siRNA / miRNA gene silencing Human 501 Mel and SK Mel 28 FANCD2

Get tips on using Corning™ Basal Cell Culture Liquid Media - DMEM and Ham's F-12, 50/50 Mix to perform Mammalian cell culture media HSG cells

Get tips on using Senescence Cells Histochemical Staining Kit to perform Reporter gene assay β-galactosidase substrates - human MSCs (mesenchymal stem cells)

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using RealTime-Glo™ Annexin V Apoptosis and Necrosis Assay to perform Necrosis LNCaP

Get tips on using RealTime-Glo™ Annexin V Apoptosis and Necrosis Assay to perform Necrosis HUVEC

Get tips on using RealTime-Glo™ Annexin V Apoptosis and Necrosis Assay to perform Necrosis A549

Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA.

Get tips on using PE anti-human CD52 Antibody to perform Flow cytometry Anti-bodies Human - CD52

Get tips on using PE anti-human CD51 Antibody to perform Flow cytometry Anti-bodies Human - CD51

Get tips on using FITC anti-human CD20 Antibody to perform Flow cytometry Anti-bodies Human - CD20