RNA isolation / purification Cells Cancer cell lines

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Human Esophagus

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Human Decidua

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Human Cornea

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Human Cerebellum

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Human Bronchi

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Human Breast

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Human Bone

Get tips on using RNeasy PowerPlant Kit (50) to perform RNA isolation / purification Plants - Seeds

Get tips on using RNeasy PowerWater Kit (50) to perform RNA isolation / purification Water samples

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.