Get tips on using Cultrex® BME Cell Invasion Assay to perform Cell migration / Invasion cell type - MDA-MB-231
Get tips on using Cultrex® Collagen IV Cell Invasion Assay to perform Cell migration / Invasion cell type - TPC1
Get tips on using LC3B Antibody Kit for Autophagy to perform Autophagy assay cell type - Human neural progenitor cells (NPC)
Get tips on using QIAamp DNA Blood Mini Kit to perform DNA isolation / purification Cells - Immortalized cell lines HEK 293T
Get tips on using Muse™Autophagy LC3-antibody based Kit to perform Autophagy assay cell type - 143B
Get tips on using STEMdiff™ Hematopoietic Kit to perform Stem cell Differentiation media Differentiation of Human iPSCs into microglia differentiation
Get tips on using EZ DNA Methylation kit to perform DNA methylation profiling Whole genome profiling - mouse T-cell (CD4 / CD8)
Get tips on using LC3B Antibody Kit for Autophagy to perform Autophagy assay cell type - Normal human fibroblasts (NHFs)
Get tips on using Annexin V-FITC Apoptosis Kit to perform Apoptosis assay cell type - Sao2-2, MG-62
When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.
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