Protein Expression Eukaryotic cells S. pastorianus

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Get tips on using Strep-Tactin Superflow Plus (10 ml) to perform Protein tag Purification of Strep-tagged proteins

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Get tips on using Ni-NTA Spin Kit (50) to perform Protein tag Detection of His-tagged proteins

Get tips on using Ni-NTA Superflow (100 ml) to perform Protein tag Purification of His-tagged proteins

Get tips on using Rat GE 4x44K v3 Microarray Kit to perform Microarray Gene expression arrays - Rat pancreas tissue Cyanine 3 & cyanine 5