Stem cell culture media

Get tips on using Anti-Clara Cell Secretory Protein Antibody to perform Flow cytometry Anti-bodies Mouse - CCSP

Get tips on using Live and Dead Cell Assay (Abcam) to perform Live / Dead assay yeast - Syspastospora parasitica

Get tips on using Live and Dead Cell Assay (Abcam) to perform Live / Dead assay yeast - Saprolegnia diclina

Get tips on using Live and Dead Cell Assay (Abcam) to perform Live / Dead assay yeast - Phytophthora parasitica

Get tips on using Live and Dead Cell Assay (Abcam) to perform Live / Dead assay bacteria - Staphylococcus aureus

Get tips on using CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) to perform Cell cytotoxicity / Proliferation assay cell type - LTEP-a-2 lung adenocarcenoma

Get tips on using Pierce™ Cell Surface Protein Isolation Kit to perform Protein isolation Mammalian cells - HUVEC

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit to perform Live / Dead assay mammalian cells - mouse, T-cell

Get tips on using ChargeSwitch™ Total RNA Cell Kit to perform RNA isolation / purification Bacteria - Gram negative Hemophilus influenzae