rna-isolation-purification-tissue-mouse-spleen

Get tips on using NucleoSpin® miRNA to perform RNA isolation / purification Cells - primary human mesenchymal stem cells

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - primary human endometrial stromal cells

Get tips on using mirVana® miRNA mimic to perform RNA isolation / purification Cells - primary human endothelial cells

Get tips on using QIAamp UltraSens Virus Kit (250) to perform RNA isolation / purification Supernatant from cell cultures

Get tips on using FastLane Cell Probe Kit (200) to perform RNA isolation / purification Cells - immortalized HT-29

Get tips on using FastLane Cell Probe Kit (200) to perform RNA isolation / purification Cells - immortalized BxPC-3

Get tips on using FastRNA™ SPIN Kit for Yeast to perform RNA isolation / purification Yeast - Pichia pastoris

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Tissue - Lamb muscle tissue

Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Tissue - Human placental tissue