dna-isolation-purification-cells-primary-cells-mouse-embryonic-fibroblast-mef

Get tips on using RNA-Bee to perform RNA isolation / purification Tissue - Mouse Colon

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Mouse Cornea

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Mouse Cerebellum

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Mouse Brainstem

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Mouse Bone

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Mouse Brain

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Cells - immortalized SK-BR-3

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Cells - immortalized ZR-75-1

Get tips on using ReliaPrep™ RNA Miniprep Systems to perform RNA isolation / purification Cells - immortalized MDA-MB-468