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RNA isolation / purification Bacteria - Gram positive Clostridium difficile Experiment

The biggest problem in isolating RNA from gram-positive bacteria is the disruption of the cell wall. A lot of protocols employ enzymatic digestion (pretreatment) which may affect gene expression patterns of certain genes. Therefore physical disruption using beads can be a best alternative.

RNA RNA isolation / purification Bacteria Gram positive Clostridium difficile

RNA isolation / purification Bacteria - Gram positive Clostridium tetani Experiment

RNA RNA isolation / purification Bacteria Gram positive Clostridium tetani

RNA isolation / purification Bacteria - Gram positive Clostridum botulinum Experiment

RNA RNA isolation / purification Bacteria Gram positive Clostridum botulinum

RNA isolation / purification Bacteria - Gram positive Lactobacillus amylovorus Experiment

RNA RNA isolation / purification Bacteria Gram positive Lactobacillus amylovorus

RNA isolation / purification Bacteria - Gram positive Listeria monocytogens Experiment

RNA RNA isolation / purification Bacteria Gram positive Listeria monocytogens

RNA isolation / purification Bacteria - Gram positive Staphylococcus epidermidis Experiment

RNA RNA isolation / purification Bacteria Gram positive Staphylococcus epidermidis

RNA isolation / purification Bacteria - Gram positive Staphylococcus saprophycitius Experiment

RNA RNA isolation / purification Bacteria Gram positive Staphylococcus saprophycitius

RNA isolation / purification Bacteria - Gram positive Streptococcus pyogenes Experiment

RNA RNA isolation / purification Bacteria Gram positive Streptococcus pyogenes

DNA isolation / purification Cells - Primary cells Mouse embryonic fibroblast (MEF) Experiment

DNA isolation and purification is the first critical step in sample preparation that helps ensure optimal performance of downstream assays like PCR, microarrays, and sequencing. Failure in yielding high-quality DNA would be the major reason that DNA doesn't work for the downstream application. To circumvent this, one should follow the recommended storage conditions to minimize DNA degradation by nucleases and shouldn't overload the purification system.

DNA DNA isolation / purification Cells Primary cells Mouse embryonic fibroblast (MEF)

RNA isolation / purification Cells - primary human primary mammary adipose derived stem cells Experiment

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

RNA RNA isolation / purification Cells primary human primary mammary adipose derived stem cells

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