siRNA / miRNA gene silencing Mouse B16-BL6

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

Get tips on using EpiTect Bisulfite Kit to perform DNA methylation profiling Whole genome profiling - mouse iPSCs

Get tips on using miRNeasy Serum/Plasma Advanced Kit (50) to perform RNA isolation / purification Tissue - Livestock Blood / Serum / Plasma / Buffy coat

Get tips on using DNeasy Blood & Tissue Kit to perform DNA isolation / purification Cells - Primary cells Mouse embryonic fibroblast (MEF)

Get tips on using Thiazolyl Blue Tetrazolium Bromide to perform Cell cytotoxicity / Proliferation assay cell type - HT22 mouse hippocampal cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Mouse Blood / serum / plasma / buffy coat

Get tips on using MLM3636 to perform CRISPR Mouse - Deletion Neuro 2a Prnp

Get tips on using JDS246 to perform CRISPR Mouse - Deletion Neuro 2a Prnp

Get tips on using lentiCRISPR to perform CRISPR Mouse - Deletion RAW 264.7 Tfeb

Get tips on using hCas9 to perform CRISPR Mouse - Deletion 3T3-L1 ATP7A