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Get tips on using RiboPure™ RNA Purification Kit, bacteria to perform RNA isolation / purification Bacteria - Gram positive Clostridium difficile
Get tips on using RiboPure™ RNA Purification Kit, bacteria to perform RNA isolation / purification Bacteria - Gram negative Salmonella enterica
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Get tips on using RiboPure™ RNA Purification Kit, bacteria to perform RNA isolation / purification Bacteria - Gram positive Staphylococcus aureus
Generally isolating RNA from Gram-negative bacteria is easy, however keeping your working environment clean and RNase free (use RNase inhibitor) is essential. Some common points to keep in mind: a) Use fresh samples for isolation or store them by freezing in RNA stabilizing buffer until use. b) Choose the bacterial input amounts carefully, to ensure buffer volumes are adequate and not to overload the columns.
Generally isolating RNA from Gram-negative bacteria is easy, however keeping your working environment clean and RNase free (use RNase inhibitor) is essential. Some common points to keep in mind: a) Use fresh samples for isolation or store them by freezing in RNA stabilizing buffer until use. b) Choose the bacterial input amounts carefully, to ensure buffer volumes are adequate and not to overload the columns.
Generally isolating RNA from Gram-negative bacteria is easy, however keeping your working environment clean and RNase free (use RNase inhibitor) is essential. Some common points to keep in mind: a) Use fresh samples for isolation or store them by freezing in RNA stabilizing buffer until use. b) Choose the bacterial input amounts carefully, to ensure buffer volumes are adequate and not to overload the columns.
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