DNA methylation profiling Gene specific profiling MCF-7

Get tips on using pSUPER.retro.puro to perform shRNA gene silencing Rat - MM1 SSH2

Get tips on using pSUPER.retro.puro to perform shRNA gene silencing Rat - MM1 LIMK1

Get tips on using pSUPER.retro.puro to perform shRNA gene silencing Rat - MM1 ADF

Get tips on using Fenozol to perform siRNA / miRNA gene silencing Human - BOSC23

Get tips on using Gentra Puregene Blood Kit to perform DNA isolation / purification Tissue - blood / plasma

Get tips on using Polybrene Infection / Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines SMMC-7721

Get tips on using QIAGEN Genomic-tip 20/G to perform DNA isolation / purification Plants - Leaves

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Get tips on using siRNA MXD3 to perform siRNA / miRNA gene silencing Human - DAOY MXD3

Get tips on using siRNA FOXS1 to perform siRNA / miRNA gene silencing Human - DAOY FOXS1