Immunofluorscence  53BP1 [H-300]

Get tips on using hCas9 to perform CRISPR Mouse - Deletion C2C12 Dmd

Get tips on using hCas9 to perform CRISPR Mouse - Deletion B117P Dck

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using Hoechst 33342 to perform Necrosis MIA PaCa-2

Get tips on using px330-mcherry to perform CRISPR Human - Repression HOTAIR

Get tips on using hCas9 to perform CRISPR Mouse - Deletion 3T3-L1 ATP7A

Get tips on using H4A3 to perform Autophagy assay cell type - SH-SY5Y

Though DNA quantification is but one small step in the multifaceted DNA sample preparation workflow, it can have large implications on the performance and validity of conclusions drawn from downstream assays. Major challenges include accuracy, precision, reproducibility, and detection of present contamination. Among UV spectrophotometry, fluorescence and real-time PCR based methods, the quantification method should be chosen based on the requirement of the downstream assay.