DNA quantification Human - SH-SY5Y

Though DNA quantification is but one small step in the multifaceted DNA sample preparation workflow, it can have large implications on the performance and validity of conclusions drawn from downstream assays. Major challenges include accuracy, precision, reproducibility, and detection of present contamination. Among UV spectrophotometry, fluorescence and real-time PCR based methods, the quantification method should be chosen based on the requirement of the downstream assay.

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1 year ago

1 year ago by Dorota Ostrowska Poland

About nuclease-free water in DNA quantification

Hi everyone! I have a question about DNA quantification. Do I need to use nuclease-free water for DNA quantification and are there any repercussions if I don’t use any?

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Protocol tips
To minimize photo bleaching
effects, keep the time for fluorescence measurement constant for all samples.

During incubation at room temperature, protected from light.
Dihydroethidium

Sigma-Aldrich

Upstream tips
This product should be kept under argon. It is both light and air sensitive. It will oxidize to ethidium on storage unless kept under argon.
Upstream tips
Do not allow pipette tip to touch the outer edges or inner sides of the wells to prevent solutions from sticking to the surface
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