siRNA / RNAi /miRNA transfection Human Cells Jurkat cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human dermal microvascular endothelial cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human coronary artery endothelial cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human aortic smooth muscle cells

Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Cells - primary human bronchial epithelial cells

Get tips on using RNAqueous®-Micro Total RNA Isolation Kit to perform RNA isolation / purification Cells - primary human epithelial cells

Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Mammalian cells - Human aortic endothelial cells

Get tips on using StemMACS™ AdipoDiff Media, human to perform Stem cell Differentiation media hMSCs differentiation into adipogenic cells

Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,