Hello everyone! I am going to do a live/dead assay for my cells and I saw that I can use both fluorescence and absorbance as my detection method. Is there a difference in the results depending on the method? Is one method preferred over the other in certain situations?
An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
Get tips on using Qubit dsDNA HS Assay Kit to perform DNA quantification Human - HeLa
Get tips on using LowCell# ChIP kit protein A x16 to perform ChIP Human - HepG2
Get tips on using EpiQuik Acetyl-Histone H4 ChIP Kit to perform ChIP Human - HepG2
Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Human - SMMC-7721
Get tips on using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® to perform RNA sequencing Rat - PC12
Get tips on using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® to perform RNA sequencing Mouse - Microglia
Get tips on using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® to perform RNA sequencing Mouse - RAW264.7
Get tips on using Fenozol to perform siRNA / miRNA gene silencing Human - BOSC23
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment