siRNA / miRNA gene silencing Human CAL-27

Get tips on using EasySep™ Human CD33 Positive Selection Kit II to perform Cell Isolation Monocyte

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

Get tips on using FITC anti-human/mouse Granzyme B Antibody to perform Flow cytometry Anti-bodies Mouse - Granzyme B

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

Get tips on using EasySep™ Release Human CD19 Positive Selection Kit to perform Cell Isolation B cell

Get tips on using EasySep™ Human CD34 Positive Selection Kit II to perform Cell Isolation CD34+ cells

Get tips on using Monoclonal Mouse Anti-Human Hepatocyte (Concentrate) Clone OCH1E5 to perform Immunohistochemistry Mouse - Hepatocyte

Get tips on using QuikChange II XL Site-Directed Mutagenesis Kit, 10 Rxn to perform Site Directed Mutagenesis (SDM) Human - Deletion K562 c-Myb gene