The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.
The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.
The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.
Get tips on using JetPrime to perform DNA transfection Mammalian cells - Immortalized cell lines HeLa
Get tips on using JetPrime to perform DNA transfection Mammalian cells - Primary cells Human chondrocytes
Get tips on using JetPrime to perform DNA transfection Mammalian cells - Immortalized cell lines COS7
Get tips on using Comet Assay Kits, 96-Well to perform DNA Damage Assay SK-OV-3
Get tips on using Comet Assay Kits, 96-Well to perform DNA Damage Assay SK-OV-3
Get tips on using Comet Assay Kits, 96-Well to perform DNA Damage Assay MIA PaCa-2
Get tips on using Comet Assay Kits, 96-Well to perform DNA Damage Assay MDA-MB-231
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment