CRISPR Mouse Deletion NIH 3T3

Get tips on using LookOut® Mycoplasma PCR Detection Kit to perform Cell Culture Contamination Detection Kit Mycoplasma

Get tips on using GeneArt™ Site-Directed Mutagenesis System to perform Site Directed Mutagenesis (SDM) Human - Point mutation THP-1 IL-33

Get tips on using Fungi-Fluor® Kit for Fungal Detection to perform Cell Culture Contamination Detection Kit Fungi

Get tips on using VetMAX™-Gold BVDV PI Detection Kit to perform Cell Culture Contamination Detection Kit Virus

Get tips on using Invitrogen™ Cell Culture Contamination Detection Kit to perform Cell Culture Contamination Detection Kit Bacteria

Get tips on using MycoAlert® Mycoplasma Detection Kit (50 Tests) to perform Cell Culture Contamination Detection Kit Mycoplasma

Get tips on using MycoAlert® Mycoplasma Detection Kit (50 Tests) to perform Cell Culture Contamination Detection Kit Mycoplasma

The process of RNA extraction from bacteria, in general, involves an RNA-protective, effective lysis of bacterial cell wall (which may pose difficulties). EDTA promotes loss of outer membrane to provide lysozyme with access to peptidoglycan. Another common method for cell wall lysis is mechanical disruption using a homogenizer (applied for gram-positive bacteria and some strains of gram-negative bacteria). Following lysis, it is necessary to disrupt protein-nucleic acid interactions, which can be achieved by adding sodium dodecyl sulfate (SDS). Next step involves using phenol-chloroform-isoamyl alcohol extraction, where RNA can be obtained from the bottom organic phase, the top phase consists of DNA and the interphase contains proteins. Isoamyl alcohol is an inert and optional addition to this mixture and is added as an anti-foaming reagent to reduce the interphase. Following RNA extraction, the samples should be checked for its quality by gel electrophoresis (23S and 16S rRNAs and 5s rRNA and tRNA bands) or UV spectrophotometric or fluorescence methods.

Get tips on using Candida albicans Detection Kits (Cat. TM34000, TM34010, EP34000) to perform Cell Culture Contamination Detection Kit Yeast

Get tips on using VetMAX™ African Swine Fever Virus Detection Kit to perform Cell Culture Contamination Detection Kit Virus