rna-isolation-purification-cells-immortalized-ntera-2

Get tips on using TRIzol™ LS Reagent to perform RNA isolation / purification Tissue - Mouse Blood / serum / plasma / buffy coat

Get tips on using QIAamp MinElute Virus Vacuum Kit (50) to perform RNA isolation / purification Viral - Viral CNS disease

Get tips on using Nucleic Acid Purification to perform Plasmid Isolation Lactococcus lactis

Get tips on using Nucleic Acid Purification to perform Plasmid Isolation DH10Bac (Bacmid)

Get tips on using Chromous Genomic DNA isolation kit to perform DNA isolation / purification Bacteria - Gram positive Bacillus subtilis

Get tips on using miRNeasy Serum/Plasma Kit to perform RNA isolation / purification Tissue - Human Blood / Serum / Plasma / Buffy coat

Get tips on using MICROBExpress™ Bacterial mRNA Enrichment Kit to perform RNA isolation / purification Bacteria - Gram positive Staphylococcus aureus

Get tips on using MICROBExpress™ Bacterial mRNA Enrichment Kit to perform RNA isolation / purification Bacteria - Gram negative Pseudomonas aeruginosa

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using QIAquick PCR Purification Kit to perform Plasmid Isolation Klebsiella pneumoniae