Flowcytometry CD3 Mouse / IgG1, kappa

Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay human - MDA-MB-231

Get tips on using CD CHO Medium to perform Mammalian cell culture media CHO

Get tips on using Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb #9733 to perform ChIP Anti-bodies H3K27me3

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized C3H-10T1/2

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized C3H-10T1/2

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - immortalized C3H-10T1/2

The process of RNA extraction from bacteria, in general, involves an RNA-protective, effective lysis of bacterial cell wall (which may pose difficulties). EDTA promotes loss of outer membrane to provide lysozyme with access to peptidoglycan. Another common method for cell wall lysis is mechanical disruption using a homogenizer (applied for gram-positive bacteria and some strains of gram-negative bacteria). Following lysis, it is necessary to disrupt protein-nucleic acid interactions, which can be achieved by adding sodium dodecyl sulfate (SDS). Next step involves using phenol-chloroform-isoamyl alcohol extraction, where RNA can be obtained from the bottom organic phase, the top phase consists of DNA and the interphase contains proteins. Isoamyl alcohol is an inert and optional addition to this mixture and is added as an anti-foaming reagent to reduce the interphase. Following RNA extraction, the samples should be checked for its quality by gel electrophoresis (23S and 16S rRNAs and 5s rRNA and tRNA bands) or UV spectrophotometric or fluorescence methods.