Select Sample type


siRNA / RNAi /miRNA transfection Rat

- Found 5253 results

Get tips on using MISSION® shRNA ZEB1 Lentiviral Transduction Particles to perform shRNA gene silencing Human - Islets of langerhans ZEB1 lentiviral particles

Products Sigma-Aldrich MISSION® shRNA ZEB1 Lentiviral Transduction Particles

Get tips on using HTRA2 MISSION shRNA Lentiviral Transduction Particles HtrA serine peptidase 2 to perform shRNA gene silencing Mouse - FL83B HtrA2

Products Sigma-Aldrich HTRA2 MISSION shRNA Lentiviral Transduction Particles HtrA serine peptidase 2

Get tips on using ShRNA CD24 Lentiviral Transduction Particles (CD24-V2LHS_71909)) to perform shRNA gene silencing Mouse - Prostate cancer cell lines (DU145 and PC3) CD24 lentiviral particles

Products Dharmacon (GE Life Sciences) ShRNA CD24 Lentiviral Transduction Particles (CD24-V2LHS_71909))

Get tips on using ShRNA CD24 Lentiviral Transduction Particles (CD24-V2LHS_71908) to perform shRNA gene silencing Mouse - Prostate cancer cell lines (DU145 and PC3) CD24 lentiviral particles

Products Dharmacon (GE Life Sciences) ShRNA CD24 Lentiviral Transduction Particles (CD24-V2LHS_71908)

Get tips on using MISSION® pLKO.1-puro Non-Mammalian shRNA Control Transduction Particles to perform shRNA gene silencing Human - Islets of langerhans Negative control (scrambled) lentiviral particles

Products Sigma-Aldrich MISSION® pLKO.1-puro Non-Mammalian shRNA Control Transduction Particles

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Proteins ELISA Mouse RANK L

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Cellular assays Reporter gene assay β-galactosidase substrates RAW 264.7

Get tips on using Ras (D2C1) Rabbit mAb #8955 to perform Western blotting Ras

Products Cell Signaling Technology Ras (D2C1) Rabbit mAb #8955

Get tips on using RIPA Buffer to perform Protein isolation Mammalian cells - Rat_Liver

Products Sigma-Aldrich RIPA Buffer

Get tips on using RIPA Buffer to perform Protein isolation Mammalian cells - Rat_Circumvallate papillae

Products Sigma-Aldrich RIPA Buffer

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms