DNA transfection Mammalian cells

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Get tips on using RPMI 1640 to perform Mammalian cell culture media HL-60

Get tips on using RPMI 1640 to perform Mammalian cell culture media CML T1

Get tips on using RPMI 1640 to perform Mammalian cell culture media A2780-CIS

Get tips on using Senescence Cells Histochemical Staining Kit to perform Cell cycle assay mouse - L929

Get tips on using DMEM HIGH GLUCOSE to perform Mammalian cell culture media PPAEC

Get tips on using MCDB 131 Medium to perform Mammalian cell culture media PCAEC

Get tips on using RPMI-1640 Medium to perform Mammalian cell culture media HUVEC

Get tips on using DMEM/F-12 to perform Mammalian cell culture media HOb

Get tips on using DMEM/F-12 to perform Mammalian cell culture media HLF