Site Directed Mutagenesis (SDM) Mouse Point mutation Neuro 2a

Get tips on using LC3B (D11) XP® Rabbit mAb to perform Autophagy assay cell type - MEFs (mouse embryonic fibroblasts)

Get tips on using Anti-LC3B antibody produced in rabbit to perform Autophagy assay cell type - MEFs (mouse embryonic fibroblasts)

Get tips on using Quick Amp Labeling Kit-one color to perform Microarray RNA amplification & Labeling - Mouse cochlaea Cyanine CTP

Get tips on using Live/Dead Cell Double Staining Kit to perform Live / Dead assay mammalian cells - L29 mouse fibroblast

Get tips on using Live and Dead Cell Assay (Abcam) to perform Live / Dead assay mammalian cells - L29 mouse fibroblast

Get tips on using TACS® 2 TdT Fluorescein Kit to perform TUNEL assay cell type - Mouse mammary gland tissue

Get tips on using CD14 Antibody, Pacific Orange™ conjugate to perform Flowcytometry CD14 - Mouse / IgG2a Human Pacific Orange™

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using Anti-Prosurfactant Protein C (proSP-C) Antibody to perform Flow cytometry Anti-bodies Mouse - proSP-C

Get tips on using T-PER™ Tissue Protein Extraction Reagent to perform Protein isolation Mammalian cells - Mouse Epididymal fat